NCI-H1770 [H1770] (ATCC® CRL-5893)

Organism: Homo sapiens, human  /  Cell Type: neuroendocrine  /  Tissue: lung; derived from metastatic site: lymph node  /  Disease: stage 4, carcinoma; non-small cell lung cancer

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Cell Type neuroendocrine
Product Format frozen
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease stage 4, carcinoma; non-small cell lung cancer
Age 57 years
Gender male
Ethnicity Caucasian
Images
Derivation
The line was established in November 1987.
Clinical Data
57 years
Caucasian, White
male
The tissue donor was a non-smoker.
Comments
A lymphoblastoid line from the same patient is available as ATCC CRL-5960.
Complete Growth Medium ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 13
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9
TPOX: 11
vWA: 18,19
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1987
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Other Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.