NCI-H1755 [H1755] (ATCC® CRL-5892™) Organism: Homo sapiens, human / Tissue: lung; derived from metastatic site: liver / Disease: stage 4, adenocarcinoma; non-small cell lung cancer General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue lung; derived from metastatic site: liver Product Format frozen Morphology Epithelial-like and/or rounded Culture Properties Adherent, with single cells and small clusters in suspension Biosafety Level 1 Disease stage 4, adenocarcinoma; non-small cell lung cancer Age 65 years Gender female Ethnicity Caucasian Derivation The line was established in October 1987. Clinical Data 65 years Caucasian female The patient was a smoker. 60 pack years. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Transfer all medium and floating cells from flask to a 50 mL centrifuge tube. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer these cells to the centrifuge tube from Step 1. Centrifuge at 125 x g for 5 to 10 minutes. Remove medium and resuspend pellet in fresh complete medium. Add appropriate aliquots of cell suspension to new culture vessels. Place culture vessels in incubators at 37°C Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Complete culture medium, 50%; FBS, 40%; DMSO, 10% Culture Conditions Temperature: 37°C STR Profile Amelogenin: XCSF1PO: 12D13S317: 10,12D16S539: 11,12D5S818: 11,12D7S820: 12F13A01: 4,6F13B: 8,10FESFPS: 11LPL: 12THO1: 7TPOX: 8vWA: 18 Name of Depositor AF Gazdar, JD Minna Year of Origin 1987 References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Restrictions The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.