NCI-H1355 [H1355] (ATCC® CRL-5865)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: pleural effusion  /  Disease: stage 4, adenocarcinoma

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleural effusion
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Disease stage 4, adenocarcinoma
Age 53 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 54; range = 43-66
Derivation The cell line was established in March 1986 from pleural effusion metastasis of lung.
Clinical Data
53 years
Caucasian
male
Complete Growth Medium ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing
Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension of the pellet. The cells grow in floating aggregates of round cell clusters.
Medium Renewal: twice weekly
Cryopreservation
Freeze medium: Complete culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: Add fresh medium as the cell density increases.
STR Profile
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 11,13
D7S820: 12
THO1: 9
TPOX: 9,12
vWA: 14,17
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.