MAVER-1 (ATCC® CRL-3008™)

Organism: Homo sapiens, human  /  Tissue: peripheral blood  /  Cell Type: lymphoblast

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Organism Homo sapiens, human
Tissue
peripheral blood
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Mantle Cell Lymphoma
Age 77 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypodiploid cell line of male origin with a chromosome number range of 38-40. The karyotype is highly rearranged and although there has been a loss of chromosome number, most of the cells still contain several of the derivative chromosomes originally described in the literature. Specifically, the t(11;14) derivative common to mantle cell lymphoma is present in a complex rearrangement with chromosome number 6, der(6)t(6;14;11)(q13;q11.2?;q23?) and was confirmed with whole chromosome paint probes for chromosomes 11 and 14. Other derivatives include add(1)(p36.1), add(4)(q31) and del(11)(q13).
Images
Clinical Data
male
Antigen Expression
CD3-, CD5+, CD10-, CD19+, CD20+, CD23-, FMC7+ (verified at ATCC)
Genes Expressed
CD3-, CD5+, CD10-, CD19+, CD20+, CD23-, FMC7+ (verified at ATCC)
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended.
Medium renewal: every 2 to 3 days depending on cell density
Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 10% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X Y
CSF1PO: 10, 11
D13S317: 8, 13
D16S539: 9, 12
D5S818: 12
D7S820: 8
THO1: 8, 9
TPOX: 8, 12
vWA: 14, 18
Population Doubling Time approximately 24 hours
Name of Depositor A Zamo
Year of Origin 2003
References

Zamo A, et al. Establishment of the MAVER-1 cell line, a model for leukemic and aggressive mantle cell lymphoma. Haematologica: 91(1):40-47, 2006. PubMed: 16434369

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cell Micrograph Other Document
Characterization Data
References

Zamo A, et al. Establishment of the MAVER-1 cell line, a model for leukemic and aggressive mantle cell lymphoma. Haematologica: 91(1):40-47, 2006. PubMed: 16434369

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365