JeKo-1 (ATCC® CRL-3006)

Organism: Homo sapiens, human  /  Tissue: peripheral blood, Mantle Cell Lymphoma  /  Disease: Lymphoma

Organism Homo sapiens, human
Tissue peripheral blood, Mantle Cell Lymphoma
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Age 78
Gender female
Applications
JeKo-1 cells are highly tumorigenic in SCID mice. RefJeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063 
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion.
Clinical Data
female
The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion.
Antigen Expression
CD3-, CD5+, CD10+, CD19+ (verified at ATCC)
Genes Expressed
CD3-, CD5+, CD10+, CD19+ (verified at ATCC)
Comments
The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. RefJeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063 
JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 104 to 4 X 104 viable cells/mL. Do not allow the cell concentration to reach 2.5 X 106 cells/mL.
Cryopreservation
Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
STR Profile
Amelogenin:X
CSF1PO:9,12
D13S317:8,9
D16S539:12
D5S818:10,13
D7S820:10,11
THO1:7
TPOX:8
vWA: 14
Name of Depositor E Campo
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

Basic Documentation
Other Documentation
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063