JVM-2 (ATCC® CRL-3002)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: Mantle Cell Lymphoma

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Mantle Cell Lymphoma
Age 63
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Bimodal chromosome number: 46 and 92This is a bimodal female cell line with approximately half the cells being pseudodiploid and the other half pseudotetraploid. Most cells contain the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes included: t(3;8)(q13;p21), add(4q) [or t(4;13)(q2?5;q1?4)], del(13)(q21) and dup(16p).
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Derivation
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Clinical Data
63
Caucasian
female
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) [PubMed: 3093393 and 3262465].
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Oncogene p53 [PubMed: 16434365]
Genes Expressed
p53 RefSalaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365,CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Comments

JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) RefMelo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393 RefMelo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465. JVM-2 cells retain p16 expression and exhibit low expression of cyclin D1. This cell line expresses cyclin D2 RefTucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118. Based on the new classification of lymphomas, JVM-2 is considered a Mantle Cell Lymphoma (MCL) RefTucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118 Refde Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187. .

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 104 to 3 x 104 viable cells/mL. Do not allow the cell concentration to exceed 1 X 106 cells/mL.
Cryopreservation
70% growth medium; 20% FBS; 10% DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17
Population Doubling Time about 48 hours
Name of Depositor J.V.Melo
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.