JVM-2 ATCC ® CRL-3002™ Homo sapiens peripheral blood Mantle

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transforme
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2
Disease	Mantle Cell Lymphoma
Age	63
Gender	female
Ethnicity	Caucasian
Applications	JVM-2 cells retain p16 expression and exhibit low expression of cyclin D1. This cell line expresses cyclin D2 [PubMed: 16183118].
Storage Conditions	liquid nitrogen vapor phase

Karyotype	Bimodal chromosome number: 46 and 92 This is a bimodal female cell line with approximately half the cells being pseudodiploid and the other half pseudotetraploid. Most cells contain the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes included: t(3;8)(q13;p21), add(4q) [or t(4;13)(q2?5;q1?4)], del(13)(q21) and dup(16p).
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Derivation	Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Clinical Data	63 Caucasian female Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) [PubMed: 3093393 and 3262465].
Antigen Expression	CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Oncogene	p53 [PubMed: 16434365]
Genes Expressed	p53 [PubMed: 16434365],CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
Comments	Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) [PubMed: 3093393 and 3262465]. JVM-2 cells retain p16 expression and exhibit low expression of cyclin D1. This cell line expresses cyclin D2 [PubMed: 16183118]. Based on the new classification of lymphomas, JVM-2 is considered a Mantle Cell Lymphoma (MCL) [PubMed: 16183118 and PubMed: 15229187].

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(4) to 3 x 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Cryopreservation	70% growth medium; 20% FBS; 10% DMSO
Culture Conditions	Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%

STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 11,13 D16S539: 12,13 D5S818: 11,12 D7S820: 10,11 THO1: 6,9 TPOX: 8,11 vWA: 17
Population Doubling Time	about 48 hours

Name of Depositor	J.V.Melo
References	Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393 Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187. Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Name of Depositor

J.V.Melo

References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph Characterization Data
References	Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393 Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187. Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

JVM-2 (ATCC® CRL-3002™)

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JVM-2 (ATCC^® CRL-3002^™)