B11F7 (ATCC® CRL-2944)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: B lymphocyte; hybridom  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type B lymphocyte; hybridom
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue.
The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species.
Storage Conditions liquid nitrogen vapor phase
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Derivation
Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function.
Genes Expressed
immunoglobulin; monoclonal antibody; against protein backbone of human and rat myelin-associated glycoprotein (MAG)
Comments
Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 10(4) to 1 X 10(5) viable cells/ml. Subculture when cell concentration is between 5 x 10(5) and 1 x 10(6) cells/ml.
Subcultivation ratio: A subcultivation ratio of 1: 5 to 1: 10 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 10% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Isotype IgG2b, kappa
Name of Depositor RH Quarles
Year of Origin 1984
References

Doberson MJ, et al. Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG). Neurochem. Res.;10(4):499-513, 1985. PubMed: 2582290

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Doberson MJ, et al. Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG). Neurochem. Res.;10(4):499-513, 1985. PubMed: 2582290