VCaP (ATCC® CRL-2876)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: prostate; derived from metastatic site: vertebral metastasis  /  Disease: cancer

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Organism Homo sapiens, human
Tissue prostate; derived from metastatic site: vertebral metastasis
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells produce the mouse xenotropic retrovirus Bxv-1]
Disease cancer
Age 59 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
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Derivation
This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo.
Clinical Data
59 years
Caucasian
male
Antigen Expression
cytokeratin-18; Homo sapiens, expressed
p53 antigen; Homo sapiens, expressed
prostate specific antigen (PSA); Homo sapiens, expressed
prostatic acid phosphatase (PAP); Homo sapiens, expressed
Rb protein; Homo sapiens, expressed
Genes Expressed
cytokeratin-18; Homo sapiens, expressed ,p53 antigen; Homo sapiens, expressed ,prostate specific antigen (PSA); Homo sapiens, expressed ,prostatic acid phosphatase (PAP); Homo sapiens, expressed ,Rb protein; Homo sapiens, expressed
Tumorigenic Yes
Effects
Yes, in nude and SCID mice
Comments
Recently, it has been shown that VCaP prostate cancer cells produce the mouse xenotropic retrovirus Bxv-1, which was likely acquired by the cells during their xenotransplantation in mice.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Hank's Balanced Salt Solution or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 2 X 104 to 4 X 104 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C.
Interval: Subculture when the cell concentration reaches between 1 X 105 and 2 X 105 cells/cm2.
Subcultivation Ratio: 1:3 to 1:4
Cryopreservation
Freeze medium: 95% growth medium; 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
Population Doubling Time about 53 hours
Name of Depositor KJ Pienta
Passage History
This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo.
Year of Origin 1997
References

Korenchuk S, et al. VCaP, a cell-based model system of human prostate cancer . In Vivo 15: 163-168, 2001. PubMed: 11317522

Knouf EC, et al. Multiple Integrated Copies and High-Level Production of the Human Retrovirus XMRV from 22Rv1 Prostate Carcinoma Cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664

Sfanos KS, et al. Identification of replication competent murine gamma retroviruses in commonly used prostate cancer cell lines. PLoS One 6:e20874, 2011.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQ's
  1. ATCC item no. CRL-2876 growth and morphology


    Date Updated: 1/30/2014

References

Korenchuk S, et al. VCaP, a cell-based model system of human prostate cancer . In Vivo 15: 163-168, 2001. PubMed: 11317522

Knouf EC, et al. Multiple Integrated Copies and High-Level Production of the Human Retrovirus XMRV from 22Rv1 Prostate Carcinoma Cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664

Sfanos KS, et al. Identification of replication competent murine gamma retroviruses in commonly used prostate cancer cell lines. PLoS One 6:e20874, 2011.