H36.12d [Pixie 12d] (ATCC® CRL-2447)

Organism: Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)  /  Cell Type: Macrophage  / 

Organism Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)
Cell Type Macrophage
Product Format frozen
Morphology macrophage
Culture Properties mixed: adherent and suspension
Biosafety Level 1
Applications
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.
Derivation
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Comments
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
  • Subculturing
    Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended
    Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
    Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Name of Depositor RT Sawyer
    Year of Origin 1991
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Basic Documentation
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.