C305 (ATCC® CRL-2424)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphoblast  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Genes Expressed
immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Cellular Products
immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Comments
Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgM kappa
Name of Depositor A Weiss
References

Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306

Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335

Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306

Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335

Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706