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Human genomic DNA (HCC2157 BL)

CRL-2341D

High molecular weight genomic DNA isolated from the HCC2157 BL EBV-transformed lymphoblastoid cell line.
Product category
Human cells
Product type
Nucleic acid
Derived from
HCC2157 BL (ATCC CRL-2341)
Organism
Homo sapiens, human
Type strain
No
Applications
Immunology
Quality control
Mass
10 μg
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
DNA Quality Control Information
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications.

Characteristics

Comments
DNA Quality Control Information
>100 kb in size by agarose gel electrophoresis
No RNA detected by agarose gel electrophoresis
A260/A280 ratio >1.8
Tested and verified for PCR amplification and restriction enzyme digestion
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications. *The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
High molecular weight DNA isolated from the HCC2157 BL EBV-transformed lymphoblastoid cell line.
Formulation: 1X TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0)
-20C or lower

Handling information

Handling notes
Resuspend in 1X TE buffer (10 mM Tris-HCL, 1 mM EDTA, pH 8.0)

Quality control specifications

Electrophoresis - RNA content
No visible RNA detected in the agarose gel
Electrophoresis - digestion
Tested and verified for PCR amplification and restriction digestion.

History

Depositors
ATCC

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

This cell line was deposited by Dr. A. Gazdar and is provided for research purposes only. This material is subject to the following restrictions in addition to those outlined in the ATCC Material Transfer Agreement:

  1. Transfers - Biological Materials may not be transferred to third parties for purposes of sale, or producing for sale.
  2. Commercial Use - all for-profit and non-profit Recipients must obtain a commercial use license prior to Commercial Use.

Any proposed Commercial Use with these cells must first be negotiated with:

The University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, Texas 75235
Telephone: (214) 648-1888
Email: [email protected]

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Need assistance with this product? Contact our Technical Support team.

Telephone

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800-638-6597

Outside the US
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