Human genomic DNA (HCC2157 BL) (ATCC® CRL-2341D™)

Organism: Homo sapiens, human  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Applications
DNA Quality Control Information
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications.
Comments
DNA Quality Control Information
>100 kb in size by agarose gel electrophoresis
No RNA detected by agarose gel electrophoresis
A260/A280 ratio >1.8
Tested and verified for PCR amplification and restriction enzyme digestion
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications. *The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
High molecular weight DNA isolated from the HCC2157 BL EBV-transformed lymphoblastoid cell line.
Formulation: 1X TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0)
-20C or lower
Biosafety Level 2
Introduction

Cell Line Description:

HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV)-transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.  HCC2157 was initiated from a 48 year old Black female with primary breast ductal carcinoma, and the DNA is available as ATCC CRL-2340D.
Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS