MB16tsA, clone 1B5 [Mbeta16tsA, clone 1B5] (ATCC® CRL-2307)

Organism: Mus musculus, mouse  /  Cell Type: fibroblastSV40 large T antigen transfected  / 

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Organism Mus musculus, mouse
Cell Type fibroblastSV40 large T antigen transfected
Product Format frozen
Morphology fibroblast
Culture Properties monolayer
Biosafety Level 2 SV-40 virus (Cells contain SV-40 viral sequences)
Age 10.5 days gestation
Gender female
Applications
MB16tsA, clone 1B5 is a fibroblast-like cell line derived from primary cultures of wild-type cells isolated from embryos from heterozygous females (beta-polymerase +/-) at day 10.5 of gestation.
The cell line is an isogenic, wild-type line to be used in concert with the base excision repair deficient cell line, MBl9tsA, clone 2B2 (ATCC CRL-2308).
Derivation
MB16tsA, clone 1B5 is a fibroblast-like cell line derived from primary cultures of wild-type cells isolated from embryos from heterozygous females (beta-polymerase +/-) at day 10.5 of gestation.
Clinical Data
MB16tsA, clone 1B5 is a fibroblast-like cell line derived from primary cultures of wild-type cells isolated from embryos from heterozygous females (beta-polymerase +/-) at day 10.5 of gestation.
female
Comments
MB16tsA, clone 1B5 is a fibroblast-like cell line derived from primary cultures of wild-type cells isolated from embryos from heterozygous females (beta-polymerase +/-) at day 10.5 of gestation.
The wild-type fibroblasts were immortalized by transfection with the DNA plasmid construct ptsA58H which contains coding sequences for both a hygromycin-resistance gene and tsA58H, a temperature-sensitive SV40 T antigen.
Cells were selected in the presence of 0.080 mg/ml hygromycin for 21 days and further selected by limiting dilution.
The cell line is an isogenic, wild-type line to be used in concert with the base excision repair deficient cell line, MBl9tsA, clone 2B2 (ATCC CRL-2308).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.08 mg/ml hygromycin B, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:15 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 34.0°C
Name of Depositor RW Sobol, SH Wilson
References

Gu H, et al. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265: 103-106, 1994. PubMed: 8016642

Sobol RW, et al. Requirement of mammalian DNA polymerase-beta in base-excision repair. Nature 379: 183-186, 1996. PubMed: 8538772

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gu H, et al. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265: 103-106, 1994. PubMed: 8016642

Sobol RW, et al. Requirement of mammalian DNA polymerase-beta in base-excision repair. Nature 379: 183-186, 1996. PubMed: 8538772