HT (ATCC® CRL-2260)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: ascites  /  Disease: diffuse mixed lymphoma

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Organism Homo sapiens, human
Tissue ascites
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease diffuse mixed lymphoma
Age 70 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Clinical Data
70 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
Genes Expressed
immunoglobulin; kappa light chain
Cellular Products
immunoglobulin; kappa light chain
Comments
HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.


Medium Renewal: 2 to 3 times a week.  

Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13,14
D16S539: 11,13
D5S818: 11,13
D7S820: 8,10
THO1: 6,7
TPOX: 11
vWA: 17,18
Isotype kappa
Name of Depositor M Beckwith
Year of Origin 1983
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723