TD.1 (ATCC® CRL-2232)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is useful for blotting of clathrin heavy chains from a variety of species.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Comments
Animals were immunized with the globular N-terminal domain of clathrin heavy chain.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful for blotting of clathrin heavy chains from a variety of species. It does not react with native clathrin in immunofluorescence or immunoprecipitation assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor FM Brodsky
References

Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490