bcd mab23 (ATCC® CRL-2107)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Derivation
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Cellular Products
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Comments
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor W Driever
References

Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244

Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244

Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245