bcd mab23 (ATCC® CRL-2107)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Derivation
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Cellular Products
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Comments
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor W Driever
References

Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244

Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244

Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245