SJSA-1 (ATCC® CRL-2098)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: osteosarcoma; multipotential sarcoma

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Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease osteosarcoma; multipotential sarcoma
Age 19 years adult
Gender male
Ethnicity Black
Storage Conditions liquid nitrogen vapor temperature
Karyotype 44XY; -1, -4, -7, -9, -11, -14, -15, +del(1)(q32;q42),+5p+, +der(7)t(4;HSR;7)(4qter-4q12::HSR::7p22--7qter), +9p+ and markers; amplified gli sequences were contained in the HSR in derivative No. 7 chromosomes
Derivation
The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.
Clinical Data
male
19 years
Black
Comments
There is amplification of the gene which encodes the p53 associated protein, MDM2.

SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Two to three times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor DN Shapiro
Year of Origin 1982
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305