T1 (174 x CEM.T1) (ATCC® CRL-1991)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast,T lymphoblast  /  Tissue: lymphoblast  / 

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Organism Homo sapiens, human
Tissue
lymphoblast
Cell Type B lymphoblast,T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr virus viral DNA sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line).
Antigen Expression
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Genes Expressed
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Comments
The cells express large amounts of HLA DR7 and HLA A2.
It does not express DR3.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539:10,12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 7, 9.3
TPOX: 8, 12
Name of Depositor P Cresswell
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841