COLO 829 (ATCC® CRL-1974)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin  /  Disease: Melanoma

Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Melanoma
Age 45 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The COLO 829 cell line was established by G.E. Moore from a biopsy specimen taken from a patient prior to chemotherapy for metastatic melanoma.

Clinical Data
45 years
Caucasian
male
Comments
A cell line was also produced from peripheral blood B cells, and is available as COLO 829BL (ATCC CRL-1980)
Early passages of COLO 829 still contain a small percentage of melanin producing cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of Trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
Name of Depositor GE Moore
Passage History
Early passages of COLO 829 still contain a small percentage of melanin producing cells.