88B (ATCC® CRL-1967)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with determinants on both the gamma and delta subunits of the receptor.
And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures.
Derivation
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica
Cellular Products
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica
Tumorigenic Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with determinants on both the gamma and delta subunits of the receptor.
And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures.
The domains recognized by 88B appear to be on the cytoplasmic side of the membrane.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor SC Froehner
References

Froehner SC, et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J. Biol. Chem. 258: 7112-7120, 1983. PubMed: 6189834

Green WN, Wanamaker CP. The role of the cystine loop in acetylcholine receptor assembly. J. Biol. Chem. 272: 20945-20953, 1997. PubMed: 9252423

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Froehner SC, et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J. Biol. Chem. 258: 7112-7120, 1983. PubMed: 6189834

Green WN, Wanamaker CP. The role of the cystine loop in acetylcholine receptor assembly. J. Biol. Chem. 272: 20945-20953, 1997. PubMed: 9252423