IL-A51 (ATCC® CRL-1871)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8.
This is one of a series of hybridomas produced by Dr.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD8
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD8
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bovine lymphocytes.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Complete Growth Medium RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor FA Osorio, WI Morrison
Deposited As mouse (B cell); mouse (myeloma)
References

Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793

Ellis JA, et al. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58: 351-358, 1986. PubMed: 2426182

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793

Ellis JA, et al. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58: 351-358, 1986. PubMed: 2426182