9-A5 (ATCC® CRL-1844)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase.
The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues.
Derivation
Animals were immunized with purified rat Na,K dependent ATPase.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase
Cellular Products
immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase
Comments
Animals were immunized with purified rat Na,K dependent ATPase.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase.
The antibody appears to bind to the alpha subunit of the enzyme.
The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgG1
    Name of Depositor HL Leffert
    References

    Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583

    Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169

    Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568

    Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099

    Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583

    Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169

    Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568

    Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099

    Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414