AGS (ATCC® CRL-1739)

Organism: Homo sapiens, human  /  Tissue: stomach  /  Disease: gastric adenocarcinoma

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Organism Homo sapiens, human
Tissue stomach
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2
Disease gastric adenocarcinoma
Age 54 years
Gender female
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.
Derivation
The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy.
Clinical Data
female
54 years
Caucasian
Tumorigenic Yes
Effects
Yes, in athymic BALB/c mice
Comments
The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection . Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile D5S818: 9, 12
D13S317: 12
D7S820: 10, 11
D16S539: 11, 13
vWA: 16, 17
TH01: 6, 7
Amelogenin: X
TPOX: 11, 12
CSF1PO: 11, 12
Population Doubling Time 20 hrs
Name of Depositor SC Barranco
Year of Origin 1979
References

Barranco SC, et al. Heterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs. Invest. New Drugs 1: 117-127, 1983. PubMed: 6678861

Barranco SC, et al. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res. 43: 1703-1709, 1983. PubMed: 6831414

Rieder G, et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128

Segal ED, et al. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc. Natl. Acad. Sci. USA 93: 1259-1264, 1996. PubMed: 8577751

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Barranco SC, et al. Heterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs. Invest. New Drugs 1: 117-127, 1983. PubMed: 6678861

Barranco SC, et al. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res. 43: 1703-1709, 1983. PubMed: 6831414

Rieder G, et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128

Segal ED, et al. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc. Natl. Acad. Sci. USA 93: 1259-1264, 1996. PubMed: 8577751