13762 MAT B III (ATCC® CRL-1666)

Organism: Rattus norvegicus, rat  /  Tissue: mammary gland  /  Disease: adenocarcinoma

Organism Rattus norvegicus, rat
Tissue mammary gland
Product Format frozen
Morphology epithelial
Culture Properties suspension or loosely adherent
Biosafety Level 1
Disease adenocarcinoma
Strain Fischer 344 (F344)
Storage Conditions liquid nitrogen vapor phase
Derivation
The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.
Comments
Prior to its deposit, the cell line was tested and found negative for the following viruses:
Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus.
The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 x 105 viable cells/mL and maintain at 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor AE Bogden
References

Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032

Basic Documentation
References

Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032