McA-RH7777 (ATCC® CRL-1601)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: hepatoma; Morris hepatoma 7777

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Organism Rattus norvegicus, rat
Tissue
liver
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent
Biosafety Level 1
Disease hepatoma; Morris hepatoma 7777
Gender female
Strain Buffalo
Applications

This cell line is a suitable transfection host.


Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
Receptor Expression
glucocorticoid
Genes Expressed
alpha-fetoprotein (AFP, alpha fetoprotein)
Comments
Addition of glucocorticoids (dexamethasone) to the medium accelerates cell proliferation and reduces alpha fetoprotein production.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Heavy monolayer sloughs off; subculture before 70% confluency. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove culture medium with floating cells to a centrifuge tube. 
  2. If any cells are attached, tap flask gently or if necessary add 2.0 to 3.0 mL of 0.25% Trypsin-0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. 
  3. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to 10 minutes. 
  5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
  6. Place culture vessels in incubators at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended
Medium Renewal: Add medium every 2 to 3 days, do not discard floating cells.
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor JE Becker
References

. . Recent Results Cancer Res. 44: 103-114, 1974.

Kulas DT, et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. PubMed: 8557682

Schock D, et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. PubMed: 8577721

Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976

Cross References

Nucleotide (GenBank) : X56145 Rat mRNA for oncofetal protein (ORF-1A).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Recent Results Cancer Res. 44: 103-114, 1974.

Kulas DT, et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. PubMed: 8557682

Schock D, et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. PubMed: 8577721

Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976