SL-29 (ATCC® CRL-1590)

Organism: Gallus gallus, chicken  /  Tissue: embryo  / 

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Organism Gallus gallus, chicken
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo; 11 days gestation
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo.
Comments
The cells have a doubling potential of 35 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: An inoculation density of 5 X 104 viable cells per cm2 of flask or dish surface area is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line.
Population Doubling Capacity The cells have a doubling potential of 35 population doublings.
Name of Depositor RJ Hay
References

. Aging in cell and tissue cultures. New York: Plenum Press; 1970.

Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

. Aging in cell and tissue cultures. New York: Plenum Press; 1970.

Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586