A2B5 clone 105 (ATCC® CRL-1520)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  /  Disease: Insulinoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Insulinoma
Applications
In the rat, A2B5 reacts with gangliosides expressed in brain, pituitary, pancreatic islets, thymic endocrine epithelium, kidney glomeruli and the adrenal medulla.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against a glycolipid antigen on neuronal cell bodies (chick, rat, bovine and human), pancreatic islet cells (rat, human, bovine), thymic epithelium (rat, human),H-2d,Increasing expression of the A2B5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F).
Cellular Products
immunoglobulin; monoclonal antibody; against a glycolipid antigen on neuronal cell bodies (chick, rat, bovine and human), pancreatic islet cells (rat, human, bovine), thymic epithelium (rat, human)
Tumorigenic Yes
Effects
Yes,
Comments
Animals were immunized with 8 day chick embryo retina cells.
Spleen cells were fused with P3X63Ag8 myeloma cells.
In the rat, A2B5 reacts with gangliosides expressed in brain, pituitary, pancreatic islets, thymic endocrine epithelium, kidney glomeruli and the adrenal medulla.
Increasing expression of the A2B5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F).
Tested and found negative for ectromelia virus (mousepox).
This line was formerly designated ATCC HB-29.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain the cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor GS Eisenbarth, M Nirenberg, FS Walsh
References

Eisenbarth GS, et al. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76: 4913-4917, 1979. PubMed: 388422

Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201

Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562

McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074

Haynes BF, et al. Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest. 71: 9-14, 1983. PubMed: 6336763

McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413

Mokhtar N, et al. Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum. Pathol. 15: 378-384, 1984. PubMed: 6609116

Eisenbarth GS, et al. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc. Natl. Acad. Sci. USA 79: 5066-5070, 1982. PubMed: 6750614

Haynes BF, et al. Demonstration of abnormalities in expression of thymic epithelial surface antigens in severe cellular immunodeficiency diseases. J. Immunol. 130: 1182-1188, 1983. PubMed: 6600476

Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Eisenbarth GS, et al. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76: 4913-4917, 1979. PubMed: 388422

Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201

Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562

McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074

Haynes BF, et al. Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest. 71: 9-14, 1983. PubMed: 6336763

McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413

Mokhtar N, et al. Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum. Pathol. 15: 378-384, 1984. PubMed: 6609116

Eisenbarth GS, et al. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc. Natl. Acad. Sci. USA 79: 5066-5070, 1982. PubMed: 6750614

Haynes BF, et al. Demonstration of abnormalities in expression of thymic epithelial surface antigens in severe cellular immunodeficiency diseases. J. Immunol. 130: 1182-1188, 1983. PubMed: 6600476

Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995