A2B5 clone 105 ATCC ® CRL-1520™ Mus musculus (B cell); Mus

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma:lymphoblast B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Disease	Insulinoma
Applications	In the rat, A2B5 reacts with gangliosides expressed in brain, pituitary, pancreatic islets, thymic endocrine epithelium, kidney glomeruli and the adrenal medulla. Tested and found negative for ectromelia virus (mousepox).

Derivation	Spleen cells were fused with P3X63Ag8 myeloma cells.
Markers	H-2d
Genes Expressed	immunoglobulin; monoclonal antibody; against a glycolipid antigen on neuronal cell bodies (chick, rat, bovine and human), pancreatic islet cells (rat, human, bovine), thymic epithelium (rat, human),H-2d,Increasing expression of the A2B5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F).
Tumorigenic	Yes
Comments	Animals were immunized with 8 day chick embryo retina cells. Spleen cells were fused with P3X63Ag8 myeloma cells. In the rat, A2B5 reacts with gangliosides expressed in brain, pituitary, pancreatic islets, thymic endocrine epithelium, kidney glomeruli and the adrenal medulla. Increasing expression of the A2B5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F). Tested and found negative for ectromelia virus (mousepox). This line was formerly designated ATCC HB-29.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain the cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype	IgM

Name of Depositor	GS Eisenbarth, M Nirenberg, FS Walsh
References	Eisenbarth GS, et al. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76: 4913-4917, 1979. PubMed: 388422 Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201 Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562 McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074 Haynes BF, et al. Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest. 71: 9-14, 1983. PubMed: 6336763 McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413 Mokhtar N, et al. Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum. Pathol. 15: 378-384, 1984. PubMed: 6609116 Eisenbarth GS, et al. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc. Natl. Acad. Sci. USA 79: 5066-5070, 1982. PubMed: 6750614 Haynes BF, et al. Demonstration of abnormalities in expression of thymic epithelial surface antigens in severe cellular immunodeficiency diseases. J. Immunol. 130: 1182-1188, 1983. PubMed: 6600476 Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995

Name of Depositor

GS Eisenbarth, M Nirenberg, FS Walsh

References

Eisenbarth GS, et al. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76: 4913-4917, 1979. PubMed: 388422

Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201

Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562

McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074

Haynes BF, et al. Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest. 71: 9-14, 1983. PubMed: 6336763

McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413

Mokhtar N, et al. Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum. Pathol. 15: 378-384, 1984. PubMed: 6609116

Eisenbarth GS, et al. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc. Natl. Acad. Sci. USA 79: 5066-5070, 1982. PubMed: 6750614

Haynes BF, et al. Demonstration of abnormalities in expression of thymic epithelial surface antigens in severe cellular immunodeficiency diseases. J. Immunol. 130: 1182-1188, 1983. PubMed: 6600476

Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

Product Sheet

Certificate of Analysis

MSDS