DBS-FRhL-2 (ATCC® CL-160™)

Organism: Macaca mulatta, monkey, rhesus  /  Tissue: lung  /  Cell Type: fibroblast

Organism Macaca mulatta, monkey, rhesus
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age fetus
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype Model number = 42. Range = 37 to 46.Seventy percent of the cells are diploid and the karyotype is that of a male rhesus monkey. No structural abnormalities are evident.
Clinical Data
male
Tumorigenic No
Comments
This line was formerly designated CCL-160.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor U.S. Government
Year of Origin April, 1969
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695