BeWo (ATCC® CCL-98)

Organism: Homo sapiens, human  /  Tissue: placenta  /  Disease: choriocarcinoma

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Organism Homo sapiens, human
Tissue
placenta
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease choriocarcinoma
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 86; range = 71 to 178.
Stemline number is hypotetraploid. Karyotype relatively stable within stemline number.
Images
Clinical Data
male
Genes Expressed
hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol. The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin
Virus Susceptibility Human poliovirus 3
Vesicular stomatitis virus
Comments
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 to 4 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16
Isoenzymes
G6PD, B
Name of Depositor RA Pattillo
References

Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554

Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575

Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667

Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001

Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422

Pattillo RA, et al. The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann. N.Y. Acad. Sci. 172: 288-298, 1971. PubMed: 5289994

Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797

Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554

Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575

Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667

Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001

Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422

Pattillo RA, et al. The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann. N.Y. Acad. Sci. 172: 288-298, 1971. PubMed: 5289994

Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797

Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185