Ch 1 Es (NBL-8) (ATCC® CCL-73)

Organism: Capra hircus, goat  / 

Organism Capra hircus, goat
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Age 2/3 term
Gender male
Applications
The cells are positive for bovine viral diarrhea virus (BVDV).
Clinical Data
male
Virus Resistance
poliovirus 3; coxsackievirus A9, B5; adenovirus 5
Comments
The cells are positive for bovine viral diarrhea virus (BVDV).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 1 to 2 times per week
Rinse cell sheet 2 twice with fresh ATV solution, remove ATV and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Basic Documentation
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438