BS-C-1 (ATCC® CCL-26™)

Organism: Cercopithecus aethiops  /  Tissue: kidney  / 

Organism Cercopithecus aethiops
Tissue
kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Normal
Applications
transfection host
detection of viruses
Storage Conditions liquid nitrogen vapor temperature
Karyotype Chromosome Frequency Distribution 100 Cells: 2n = 60 One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes.
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Comments
The cells are positive for keratin by immunoperoxidase staining.
African green monkey kidney cell line suitable for transfection by SV40 vectors.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor HE Hopps
References

Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327

Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327

Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.