KG-1 (ATCC® CCL-246™)

Organism: Homo sapiens, human  /  Tissue: bone marrow  /  Cell Type: Macrophage

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Organism Homo sapiens, human
Tissue
bone marrow
Cell Type Macrophage
Product Format frozen
Morphology myeloblast
Culture Properties suspension
Biosafety Level 1
Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations.
Derivation
The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia.
Clinical Data
59 years
A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia.
Caucasian
male
Antigen Expression
HLA A30, A31, B35, Cw4
Genes Expressed
HLA DR,HLA A30, A31, B35, Cw4
Comments
KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells.
They show a good response to colony stimulating factor (CSF).
The line is EBNA negative (EBNA-).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow to exceed 2 X 10(6) cells/ml.
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Name of Depositor DW Golde
References

Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

. . Blood 54: 174a, 1979.

Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689

Cross References

Nucleotide (GenBank) : AF146432 Homo sapiens cell-line KG-1a sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

. . Blood 54: 174a, 1979.

Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689