M-MSV-BALB/3T3 (ATCC® CCL-163.2)

Organism: Mus musculus, mouse  /  Cell Type: fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed  / 

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Organism Mus musculus, mouse
Cell Type fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 14 to 17 day gestation embryo
Strain BALB/c
Applications
Do not produce M-MSV.
Derived from BALB/3T3 (CCL-163).
Derivation
Derived from BALB/3T3 (CCL-163).
Antigen Expression
H-2d
Genes Expressed
H-2d
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Do not produce M-MSV.
Derived from BALB/3T3 (CCL-163).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Aaronson
References

Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987