RPMI 1788 (ATCC® CCL-156)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  / 

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 33 years
Gender male
Ethnicity Caucasian
Karyotype human male; hypodiploid; stable
Clinical Data
male
Caucasian
33 years
Antigen Expression
HLA A2, Aw33, B7, B14
Genes Expressed
immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta),HLA A2, Aw33, B7, B14
Cellular Products
immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)
Comments
The cells are EBNA positive.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium.Cultures are established at 3 X 10 exp5 viable cells/ml.
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Isoenzymes
G6PD, B
Isotype IgM; lambda light chain
Name of Depositor GE Moore
References

Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523

Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807

Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523

Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807

Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241