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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Species
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Cryptosporidium parvum Tyzzer
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Origin
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cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves cryptosporidiosis
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Zap express
Intact vector size: 38.937
Type of vector: phage
Cloning sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Polylinker sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Cloning capacity: 12 kb
Construction: pAD-GAL4
Features (with orientation and position when available):
enhancer: CMV, SV40
marker(s): neoR, kanR
terminator: SV40, Tk
promoter: CMV, T3, T7
insert detection: lacZ
replicon: pMB1, f1, lamda
Cross references:
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Vector
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Tissue: cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves DNA: genomic Digest: Partial Insert size range: 0.5-5.0 kb Coverage: Clones for "complete" coverage: 62500.0
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Shipping Information
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Distributed: frozen bacteria-free lysate
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Comments
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Label on vial may be incorrect. This is a GENOMIC library, not a cDNA library.
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References
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Arrowood MJ, et al. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice. Infect. Immun. 57: 2283-2288, 1989. PubMed: 2744847
Arrowood MJ, Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 73: 314-319, 1987. PubMed: 3585626
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