IOWA 395 (ATCC® 87760)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: C Dykstra

Permits and Restrictions

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Designations IOWA 395
Species Cryptosporidium parvum Tyzzer
Depositors C Dykstra
Origin
cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves
cryptosporidiosis
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Zap express
Intact vector size: 38.937
Type of vector: phage
Cloning sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Polylinker sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Cloning capacity: 12 kb
Construction: pAD-GAL4
Features (with orientation and position when available):
enhancer: CMV, SV40
marker(s): neoR, kanR
terminator: SV40, Tk
promoter: CMV, T3, T7
insert detection: lacZ
replicon: pMB1, f1, lamda
Cross references:
Vector
Tissue: cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves
DNA: genomic
Digest: Partial
Insert size range: 0.5-5.0 kb
Coverage: Clones for "complete" coverage: 62500.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate
Comments
Label on vial may be incorrect. This is a GENOMIC library, not a cDNA library.
References

Arrowood MJ, et al. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice. Infect. Immun. 57: 2283-2288, 1989. PubMed: 2744847

Arrowood MJ, Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 73: 314-319, 1987. PubMed: 3585626

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