IOWA 395 (ATCC® 87760)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: C Dykstra

Permits and Restrictions

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Designations IOWA 395
Species Cryptosporidium parvum Tyzzer
Depositors C Dykstra
Origin
cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves
cryptosporidiosis
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Zap express
Intact vector size: 38.937
Type of vector: phage
Cloning sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Polylinker sites: SacI SalI SpeI BamHI EcoRI HindIII XhoI XbaI NotI ApaI SmaI
KpnI
Cloning capacity: 12 kb
Construction: pAD-GAL4
Features (with orientation and position when available):
enhancer: CMV, SV40
marker(s): neoR, kanR
terminator: SV40, Tk
promoter: CMV, T3, T7
insert detection: lacZ
replicon: pMB1, f1, lamda
Cross references:
Vector
Tissue: cryptosporidium oocysts and sporozoites obtained from the feces of infected Holstein calves
DNA: genomic
Digest: Partial
Insert size range: 0.5-5.0 kb
Coverage: Clones for "complete" coverage: 62500.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate
Comments
Label on vial may be incorrect. This is a GENOMIC library, not a cDNA library.
References

Arrowood MJ, et al. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice. Infect. Immun. 57: 2283-2288, 1989. PubMed: 2744847

Arrowood MJ, Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 73: 314-319, 1987. PubMed: 3585626

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