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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
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Vector
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Tissue: sporozoites/oocysts DNA: genomic Digest: Complete Insert size range: 0.2 - 2.0 Coverage: Clones for "complete" coverage: 16000.0
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Comments
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The 95-400 isolate was collected from the feces of a two year old beef steer in Ada County, Idaho. Oocysts were purified from feces on CsCl gradients and surface sterilized with 10% Clorox prior to nucleic acid extraction.
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References
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Nikolai V Khramtsov, personal communication
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