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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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Schizosaccharomyces pombe General Purpose Vectors
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Depositors
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H Okayama, A Ahmed, SL Forsburg, JB Keeney, G Cottarel, NC Barbet, AM Carr
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Vector Information
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Vectors included are:
| Designation |
ATCC No. |
Features |
Reference |
| pAL19 |
37798 |
LEU2 blue/white |
1 |
| pBG1 |
87035 |
his3+ blue/white |
5 |
| pEA2 |
87617 |
his7+ in E. coli |
3 |
| pEA500 |
87618 |
his7+ in S. pombe episome with ars1 |
3 |
| pJK148 |
86956 |
leu1+ blue/white integrating |
6 |
| pJK210 |
86957 |
ura4+ blue/white integrating |
6 |
| pSP1 |
77497 |
LEU2 |
4 |
| pSP2 |
77498 |
URA3 |
4 |
| pUR18 |
77297 |
ura4+ blue/white |
2 |
| pUR19 |
77298 |
ura4+ blue/white |
2 |
Plate Map:
| Well |
ATCC No. |
Designation |
Selection |
| A1 |
37798 |
pAL19 |
tetR |
| A2 |
87035 |
pBG1 |
ampR |
| A3 |
87617 |
pEA2 |
ampR |
| A4 |
87618 |
pEA500 |
ampR |
| A5 |
86956 |
pJK148 |
ampR |
| A6 |
86957 |
pJK210 |
ampR |
| A7 |
77497 |
pSP1 |
ampR |
| A8 |
77498 |
pSP2 |
ampR |
| A9 |
77297 |
pUR18 |
ampR |
| A10 |
77298 |
pUR19 |
ampR |
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Comments
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A set of general purpose shuttle vectors for Schizosaccharomyces pombe.
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References
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Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111
Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169
Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485
Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314
Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439
Ahmed A. Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis. Gene 141: 71-73, 1994. PubMed: 8163177
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