Schizosaccharomyces pombe General Purpose Vectors (ATCC® 87686)

Depositors: H Okayama, A Ahmed, SL Forsburg, JB Keeney, G Cottarel, NC Barbet, AM Carr

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Designations Schizosaccharomyces pombe General Purpose Vectors
Depositors H Okayama, A Ahmed, SL Forsburg, JB Keeney, G Cottarel, NC Barbet, AM Carr
Biosafety Level 1
Vector Information Vectors included are:

 Designation ATCC No.  Features  Reference 
 pAL19  37798  LEU2 blue/white  1
 pBG1  87035  his3+ blue/white  5
 pEA2  87617  his7+ in E. coli  3
 pEA500  87618  his7+ in S. pombe episome with ars1  3
 pJK148  86956  leu1+ blue/white integrating  6
 pJK210  86957  ura4+ blue/white integrating  6
 pSP1  77497  LEU2  4
 pSP2  77498  URA3  4
 pUR18  77297  ura4+ blue/white  2
 pUR19  77298  ura4+ blue/white  2

 

Plate Map:

 Well ATCC No.  Designation Selection 
A1  37798  pAL19 tetR
 A2  87035  pBG1  ampR
 A3  87617  pEA2 ampR
A4  87618 pEA500  ampR
A5  86956  pJK148  ampR
 A6  86957 pJK210  ampR
 A7  77497 pSP1  ampR
A8  77498  pSP2  ampR
 A9  77297  pUR18  ampR
 A10  77298  pUR19  ampR

Comments
A set of general purpose shuttle vectors for Schizosaccharomyces pombe.
References

Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111

Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314

Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439

Ahmed A. Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis. Gene 141: 71-73, 1994. PubMed: 8163177

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