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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 6.5019998550415040 Vector: pWE3 (phagemid) Promoters: Promoter for in vitro transcription SP6 Marker(s):ampR,purR Construct size (kb): 6.501999855041504 Features: marker(s): ampR
marker(s): puromycin resistance
promoter for expression: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40
replicon: f1
replicon: pMB1
MCS: HindIII...ApaI
terminator: SV40 polyadenylation
enhancer: SV40
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--6.5; SalI--3.6, 2.9. Having identical cloning sites also facilitates shuttling cDNAs from one vector to another. One of a series of vectors ( ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available ( ATCC 87678). Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits.
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References
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Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206
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Shipping Information
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Freeze dried E. coli containing the plasmid
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