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Yeast Shuttle Expression Vectors (ATCC^® 87669^™)

Depositors: Martin Funk, Medigene GmbH, Martinsried/Munchen, Germany

Yeast Shuttle Expression Vectors ATCC^® 87669^™

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For-Profit: $310.00 Non-Profit: $310.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	Yeast Shuttle Expression Vectors
Depositors	Martin Funk, Medigene GmbH, Martinsried/Munchen, Germany
Biosafety Level	1
Vector Information	ATCC^® Catalog No. 87354 Designation: p413 GPD plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (kb): 5.876 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: SalIXbaI, ->, 2343-2401 Promoter for expression: GPD, <-, 2401-3081 Marker: ampR, <-, 4328-5258 Replicon: CEN6/ARSH4, ->, 5363-5876 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.2, 0.7; EcoRI - 5.9; BamHI - 5.9. ATCC^® Catalog No. 87355 Designation: p423 GPD plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (kb): 6.709 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2337-2401 Promoter for expression: GPD, <-, 2401-3081 Marker: ampR, <-, 4233-5163 Replicon: 2 micron, ->, 5262-6602 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 0.7; EcoRI - 6.7; BamHI - 6.7. ATCC^® Catalog No. 87356 Designation: p414 GPD plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (in kb): 5.694 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, ->, 200-1213 Terminator: CYC1, ->, 1895-2155 MCS: XhoIXbaI, ->, 2155-2219 Promoter for expression: GPD, <-, 2219-2899 Marker: ampR, <-, 4146-5076 Replicon: CEN6/ARSH4, ->, 5181-5694 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 1.9, 0.7; EcoRI - 5.8; BamHI - 5.8. ATCC^® Catalog No. 87357 Designation: p424 GPD plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 6.527 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, -> Terminator: CYC1, -> Promoter for expression: GPD, <- Marker: ampR, <- Replicon: 2 micron, -> Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 1.7, 1.0, 0.68; EcoRI - 6.5; BamHI - 6.5. ATCC Catalog No. 87358 Designation: p415 GPD plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size(in kb): 6.927 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3388-3452 Promoter for expression: GPD, <-, 3452-4132 Marker: ampR, <-, 5379-6309 Replicon: CEN6/ARSH4, ->, 6141-6927 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 6.2, 0.7; EcoRI - 4.6, 2.3; BamHI - 6.9. ATCC^® Catalog No. 87359 Designation: p425 GPD plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 7.760 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI Features (with orientation and position when available):Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3388-3452 Promoter for expression: GPD, <-, 3452-4132 Marker: ampR, <-, 5284-6214 Replicon: 2 micron, ->, 6313-7653 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.9, 3.2, 0.7; EcoRI - 5.5, 2.3; BamHI - 7.8. ATCC Catalog No. 87360 Designation: p416 GPD plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (in kb): 5.804 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: URA3, -> Terminator: CYC1, -> Promoter for expression: GPD, <- Marker: ampR, <- Replicon: CEN6/ARSH4, -> Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.13, 0.68; EcoRI - 5.8; BamHI - 5.8. ATCC^® Catalog No. 87361 Designation: p426 GPD plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 6.637 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: URA3, ->, 200-1300 Terminator: CYC1, ->, 2004-2264 MCS: XhoIXbaI, ->, 2265-2339 Promoter for expression: GPD, <-, 2339-3019 Marker: ampR, <-, 4138-5068 Replicon: 2 micron, ->, 5167-6507 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 0.7; EcoRI - 6.6; BamHI - 6.6. ATCC^® Catalog No. 87362 Designation: p413 TEF plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (in kb): 5.598 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2337-2400 Promoter for expression: TEF, <-, 2401-2803 Marker: ampR, <-, 4050-4980 Replicon: CEN6/ARSH4, ->, 5084-5598 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.2, 0.4; XhoI - 5.6; EcoRI - 5.6; BamHI - 5.6. ATCC^® Catalog No. 87363 Designation: p423 TEF plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 6.431 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2337-2401 Promoter for expression: TEF, <-, 2401-2803 Marker: ampR, <-, 3954-4884 Replicon: 2 micron, ->, 4984-6324 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 0.42; BamHI - 6.4; XhoI - 6.4. ATCC^® Catalog No. 87364 Designation: p414 TEF plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (in kb): 5.416 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, ->, 200-1213 Terminator: CYC1, ->, 1895-2155 MCS: XhoIXbaI, ->, 2161-2219 Promoter for expression: TEF, <-, 2219-2621 Marker: ampR, <-, 3868-4798 Replicon: CEN6/ARSH4, ->, 4902-5416 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.1, 1.7, 0.42; EcoRI - 5.6; BamHI - 5.6. ATCC^® Catalog No. 87365 Designation: p424 TEF plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 6.249 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, ->, 200-1213 Terminator: CYC1, ->, 1895-2155 MCS: XhoIXbaI, ->, 2155-2219 Promoter for expression: TEF, <-, 2219-2621 Marker: ampR, <-, 3772-4702 Replicon: 2 micron, ->, 4802-6142 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 1.7, 1.0, 0.44; BamHI - 6.2; EcoRI - 6.2. ATCC^® Catalog No. 87366 Designation: p415 TEF plasmid in E. coli Description: Low copy number shuttle expression vector. Vector size (in kb): 6.649 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3388-3452 Promoter for expression: TEF, <-, 3452-3854 Marker: ampR, <-, 5101-6031 Replicon: CEN6/ARSH4, ->, 6135-6649 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 6.2, 0.42; XhoI - 6.6; BamHI - 6.6. ATCC^® Catalog No. 87367 Designation: p425 TEF plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 5.526 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoISpeI, ->, 3388-3445 Promoter for expression: TEF, <-, 3452-3854 Marker: ampR, <-, 5005-5935 Replicon: 2 micron, ->, 6035-7375 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.8, 3.2, 0.42; BamHI - 7.8; EcoRI - 5.0, 2.4. ATCC^® Catalog No. 87368 Designation: p416 TEF plasmid in E. coli Description: Low copy number shuttle expression vector Vector size(in kb): 5.526 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: URA3, ->, 200-1323 Terminator: CYC1, ->, 2005-2265 MCS: XhoIXbaI, ->, 2265-2328 Promoter for expression: TEF, <-, 2329-2731 Marker: ampR, <-, 3978-4908 Replicon: CEN6/ARSH4, ->, 5012-5526 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.2, 0.42; BamHI - 5.6; EcoRI - 5.6. ATCC^® Catalog No. 87369 Designation: p426 TEF plasmid in E. coli Description: High copy number shuttle expression vector. Vector size (in kb): 6.359 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: URA3, ->, 200-1300 Terminator: CYC1, ->, 2004-2264 MCS: XhoIXbaI, ->, 2265-2339 Promoter for expression: TEF, <-, 2339-2741 Marker: ampR, <-, 3859-4789 Replicon: 2 micron, ->, 4889-6229 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.7, 0.44; BamHI - 6.5; XhoI - 6.5. ATCC^® Catalog No. 87370 Designation: p413 ADH plasmid in E. coli Description: Low copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size (in kb): 6.696 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2337-2400 Promoter for expression: ADH, <-, 2401-3901 Marker: ampR, <-, 5148-6078 Replicon: CEN6/ARSH4, ->, 6183-6696 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.2, 1.6; BamHI - 6.8; XhoI - 6.8. ATCC^® Catalog No. 87371 Designation: p423 ADH plasmid in E. coli Description: High copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size (in kb): 7.529 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2076-2401 Promoter for expression: ADH, <-, 2401-3901 Marker: ampR, <-, 5053-5983 Replicon: 2 micron, ->, 6082-7422 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 1.5; BamHI - 7.6; EcoRI - 7.6. ATCC^® Catalog No. 87372 Designation: p414 ADH plasmid in E. coli Description: Low copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size: 6.514 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, ->, 200-1213 Terminator: CYC1, ->, 1895-2155 MCS: XhoIXbaI, ->, 2155-2219 Promoter for expression: ADH, <-, 2219-3719 Marker: ampR, <-, 4966-5896 Replicon: CEN6/ARSH4, ->, 6001-6514 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 1.8, 1.5; BamHI - 6.6; EcoRI - 6.6; XhoI - 6.6. ATCC^® Catalog No. 87373 Designation: p424 ADH plasmid in E. coli Description: High copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size(in kb): 7.347 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, ->, 200-1213 Terminator: CYC1, ->, 1895-2155 MCS: XhoIXbaI, ->, 2155-2219 Promoter for expression: ADH, <-, 2219-3719 Marker: ampR, <-, 4871-5801 Replicon: CEN6/ARSH4, ->, 5900-7240 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 1.7, 1.5, 1.0; BamHI - 7.4; EcoRI - 7.4. ATCC^® Catalog No. 87374 Designation: p415 ADH plasmid in E. coli Description: Low copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size (kb): 7.747 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3388-3452 Promoter for expression: ADH, <-, 3452-4952 Marker: ampR, <-, 6199-7129 Replicon: CEN6/ARSH4, ->, 7234-7747 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 6.0, 1.5; EcoRI - 5.4, 2.3; KpnI - 5.8, 10.7. ATCC^® Catalog No. 87376 Designation: p416 ADH plasmid in E. coli Description: Low copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Vector size (in kb): 6.624 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: URA3, ->, 200-1323 , 2005-2265 MCS: XhoIXbaI, ->, 2265-2328 Promoter for expression: ADH, <-, 2329-3829 Marker: ampR, <-, 5076-6006 Replicon: CEN6/ARSH4, ->, 6111-6624 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.1, 1.5; KpnI - 6.6; EcoRI - 6.6. ATCC^® Catalog No. 87377 Designation: p426 ADH plasmid in E. coli Description: High copy number shuttle expression vector. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources Vector size (in kb): 7.457 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: URA3, ->, 200-1300 Terminator: CYC1, ->, 2004-2264 MCS: XhoIXbaI, ->, 2265-2339 Promoter for expression: ADH, <-, 2339-3839 Marker: ampR, <-, 4958-5888 Replicon: CEN6/ARSH4, ->, 5987-7327 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 1.5; EcoRI - 7.4; XbaI - 4.6, 2.7. ATCC^® Catalog No. 87378 Designation: p413 CYC1 plasmid in E. coli Description: Low copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 5.483 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2007-2337 MCS: SalIXbaI, ->, 2343 - 2400 Promoter for expression: CYC1, <-, 2400-2687 Marker: ampR, <-, 3935-4865 Replicon: CEN6/ARSH4, ->, 4969-5483 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.2, 0.3; EcoRI - 5.5; KpnI - 4.5, 0.9. ATCC^® Catalog No. 87379 Designation: p423 CYC1 plasmid in E. coli Description: High copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 6.316 Type of vector: plasmid Markers: ampR, HIS3 Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: HIS3, ->, 200-1395 Terminator: CYC1, ->, 2077-2337 MCS: XhoIXbaI, ->, 2343-2400 Promoter for expression: CYC1, <-, 2400-2687 Marker: ampR, <-, 3819-4749 Replicon: 2 micron, ->, 4869-6209 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.8, 0.35; XbaI - 3.4, 2.9; HindIII - 4.8, 1.4. ATCC^® Catalog No. 87380 Designation: p414 CYC1 plasmid in E. coli Description: Low copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 5.301 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: SalI ClaI EcoRI PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, -> Terminator: CYC1, -> MCS: XhoIXbaI, -> Promoter for expression: CYC1, <- Marker: ampR, <- Replicon: CEN6/ARSH4, -> Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.4, 1.7, 0.3; BamHI - 5.6; EcoRI - 5.6. ATCC^® Catalog No. 87381 Designation: p424 CYC1 plasmid in E. coli Description: High copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 6.134 Type of vector: plasmid Markers: ampR, TRP1 Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: TRP1, -> Terminator: CYC1, -> MCS: XhoIXbaI, -> Promoter for expression: CYC1, <- Marker: ampR, <- Replicon: 2 micron, -> Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.6, 1.8, 1.1, 0.3; XbaI - 3.5, 1.6, 0.94; BamHI - 6.6; EcoRI - 6.6; SacI - 6.2. ATCC^® Catalog No. 87382 Designation: p415 CYC1 plasmid in E. coli Description: Low copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 6.534 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: SalI HindIII PstI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3394-3452 Promoter for expression: CYC1, <-, 3452-3739 Marker: ampR, <-, 4986-5916 Replicon: CEN6/ARSH4, ->, 6000-6514 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 6.2, 0.3; KpnI - 5.0, 1.6; EcoRI - 4.3, 2.3. ATCC Catalog No. 87383 Designation: p425 CYC1 plasmid in E. coli Description: High copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 7.367 Type of vector: plasmid Markers: ampR, LEU2 Cloning sites: SalI HindIII PstI SmaI BamHI SpeI Features (with orientation and position when available): Marker: LEU2, ->, 200-2446 Terminator: CYC1, ->, 3128-3388 MCS: XhoIXbaI, ->, 3394-3452 Promoter for expression: CYC1, <-, 3452-3739 Marker: ampR, <-, 4870-5800 Replicon: 2 micron, ->, 5920-7260 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.9, 3.2, 0.35; XbaI - 3.9, 3.5; KpnI - 6.0, 1.4. ATCC^® Catalog No. 87384 Designation: p416 CYC1 plasmid in E. coli Description: Low copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 5.411 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Features (with orientation and position when available): Marker: URA3, ->, 200-1323 Terminator: CYC1, ->, 2005-2265 MCS: SalIXbaI, ->, 2271-2328 Promoter for expression: CYC1, <-, 2329-2616 Marker: ampR, <-, 3863-4793 Replicon: CEN6/ARSH4, ->, 4887-5391 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 5.1, 0.3; KpnI - 5.4; EcoRI - 5.4. ATCC^® Catalog No. 87385 Designation: p426 CYC1 plasmid in E. coli Description: High copy number shuttle expression vector. Contains a truncated version of the CYC1 promoter that is very weak and is no longer inducible because of a deletion of most of the UAS2 sequence. Vector size (in kb): 6.244 Type of vector: plasmid Markers: ampR, URA3 Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI SpeI Features (with orientation and position when available): Marker: URA3, ->, 200-1300 Terminator: CYC1, ->, 2004-2264 MCS: SalIXbaI, ->, 2271-2339 Promoter for expression: CYC1, <-, 2339-2626 Marker: ampR, <-, 3724-4654 Replicon: 2 micron, ->, 4774-6114 Restriction digests of the vector give the following sizes (in kb): SacI/XbaI - 3.2, 2.75. 0.35; XbaI - 3.4, 2.9; EcoRI - 6.3.
Comments	NOTE: ATCC® 87375™, p425 ADH is no longer available and is not distributed in this kit. A set of 31 yeast expression vectors (ATCC 87354-87374, 87376 -87385) differing in promoter (GPD, TEF, ADH, CYC1), selection marker (HIS3, TRP1, LEU2, URA3) and replicon (CEN6/ARSH4, 2 micron).
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37°C
References	Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
Related Products	Component: ATCC 87354 Component: ATCC 87355 Component: ATCC 87356 Component: ATCC 87357 Component: ATCC 87358 Component: ATCC 87359 Component: ATCC 87360 Component: ATCC 87361 Component: ATCC 87362 Component: ATCC 87363 Component: ATCC 87364 Component: ATCC 87365 Component: ATCC 87366 Component: ATCC 87367 Component: ATCC 87368 Component: ATCC 87369 Component: ATCC 87370 Component: ATCC 87371 Component: ATCC 87372 Component: ATCC 87373 Component: ATCC 87374 Component: ATCC 87376 Component: ATCC 87377 Component: ATCC 87378 Component: ATCC 87379 Component: ATCC 87380 Component: ATCC 87381 Component: ATCC 87382 Component: ATCC 87383 Component: ATCC 87384 Component: ATCC 87385
Shipped	FZ
Shipping Information	Frozen glycerol stocks of Escherichia coli containing plasmid arrayed on a microtiter plate.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector Map ATCC 87354 Vector map ATCC 87355 Vector Map ATCC 87356 Vector Map ATCC 87357 Vector Map ATCC 87358 Vector Map ATCC 87359 Vector Map ATCC 87360 Vector Map ATCC 87361 Vector Map ATCC 87362 Vector Map ATCC 87363 Vector Map ATCC 87364 Vector Map ATCC 87365 Vector Map ATCC 87366 Vector Map ATCC 87367 Vector Map ATCC 87368 Vector Map ATCC 87369 Vector Map ATCC 87370 Vector Map ATCC 87371 Vector Map ATCC 87372 Vector Map ATCC 87373 Vector Map ATCC 87374 Vector Map ATCC 87376 Vector Map ATCC 87377 Vector Map ATCC 87378 Vector Map ATCC 87379 Vector Map ATCC 87380 Vector Map ATCC 87381 Vector Map ATCC 87382 Vector Map ATCC 87383 Vector Map ATCC 87384 Vector Map ATCC 87385

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p423 GPD (ATCC^® 87355^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p414 GPD (ATCC^® 87356^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p424 GPD (ATCC^® 87357^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p415 GPD (ATCC^® 87358^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p425 GPD (ATCC^® 87359^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p416 GPD (ATCC^® 87360^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p426 GPD (ATCC^® 87361^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p413 TEF (ATCC^® 87362^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p423 TEF (ATCC^® 87363^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p414 TEF (ATCC^® 87364^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p424 TEF (ATCC^® 87365^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p415 TEF (ATCC^® 87366^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p425 TEF (ATCC^® 87367^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p416 TEF (ATCC^® 87368^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p426 TEF (ATCC^® 87369^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p413 ADH (ATCC^® 87370^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p423 ADH (ATCC^® 87371^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p414 ADH (ATCC^® 87372^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p424 ADH (ATCC^® 87373^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p415 ADH (ATCC^® 87374^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p416 ADH (ATCC^® 87376^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p426 ADH (ATCC^® 87377^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p413 CYC1 (ATCC^® 87378^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p423 CYC1 (ATCC^® 87379^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p414 CYC1 (ATCC^® 87380^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p424 CYC1 (ATCC^® 87381^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p415 CYC1 (ATCC^® 87382^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p425 CYC1 (ATCC^® 87383^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

p416 CYC1 (ATCC^® 87384^™)

frozen

For-Profit: $290.00 Non-Profit: $290.00

p426 CYC1 (ATCC^® 87385^™)

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00