pCM185 (ATCC® 87659)

Applications: YC-type (centromeric) shuttle vectorexpression vector  /  Depositors: E Herrero

Designations pCM185
Depositors E Herrero
Biosafety Level 1
Vector Information
Size (kb): 7.7300000190734860
Vector: pCM185 (plasmid)
Promoters: Promoter CMV
Marker(s):TRP1,ampR
Construct size (kb): 7.730000019073486
Features: marker(s): TRP1
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; PstI--7.8; BamHI/XhoI--6.9, 0.8.
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672

Related Products
component of:ATCC 87663
Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87659