YEpFAT7 (ATCC® 87645)

Applications: Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.  /  Depositors: K Runge

Permits and Restrictions

View Permits

Designations YEpFAT7
Depositors K Runge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 10.3179998397827200
DESCRIPTION OF VECTOR:
Intact vector size: 10.318
Type of vector: plasmid
Cloning sites: SacI SmaI SalI
Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII
Other unique sites: NotI
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 9016-9035
MCS: EcoRI...HindIII, ->, 10269-2
marker(s): GART, <-, 2-2091
replicon: 2 micron/LEU2-d, 2086-4780
marker(s): LEU2-d, ->, 3443-4537
marker(s): URA3, <-, 4860-5662
promoter for in vitro transcription: T3, <-, 5895-5914
promoter: lac, <-, 5984-6012
replicon: pMB1, 6338-6338
marker(s): ampR, <-, 7096-7956
Vector: YEpFAT7 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):URA3,ampR,LEU2-d
Construct size (kb): 10.31799983978272
Features: marker(s): GART
marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: EcoRI...HindIII
Applications
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.6, 1.0; SmaI--11.0; HindIII--6.2, 4.8.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media M9 + tryptophan + leucine
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried