YEpFAT4 (ATCC® 87642)

Applications: M9 + tryptophan medium recommended for DNA purification.Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT4
Depositors K Runge
Biosafety Level 1
Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 7.0110001564025880
DESCRIPTION OF VECTOR:
Intact vector size: 7.011
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 860-879
MCS: NotI...XbaI, ->, 908-942
replicon: 2 micron/LEU2-d, 941-3635
marker(s): LEU2-d, ->, 2298-3392
marker(s): URA3, <-, 3715-4517
restriction site: HindIII, 4740-4740
promoter for in vitro transcription: T3, <-, 4750-4769
promoter: lac, <-, 4839-4867
replicon: pMB1, 5193-5193
marker(s): ampR, <-, 5951-6811
Vector: YEpFAT4 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):URA3,ampR,LEU2-d
Construct size (kb): 7.011000156402588
Features: marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII
Applications
M9 + tryptophan medium recommended for DNA purification.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.1; SmaI--7.1; EcoRI--5.0, 2.1.
M9 + tryptophan medium recommended for DNA purification.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media M9 + tryptophan + leucine
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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