pCMVtkLUC+ (ATCC® 87633)

Applications: reporter construct  shuttle vector  /  Depositors: J Duschl, J Altschmied

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Designations pCMVtkLUC+
Depositors J Duschl, J Altschmied
Biosafety Level 1
Vector Information
Size (kb): 5.3759999275207520
Vector: pCMVtkLUC+ (plasmid)
Promoters: Promoter TK
Marker(s):ampR
Construct size (kb): 5.375999927520752
Features: marker(s): ampR
promoter: TK
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...SacI
MCS: HindIII, SalI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
enhancer: CMV
Applications
reporter construct
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 0.6; EcoRI--3.2, 2.2.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The vector contains the human cytomegalovirus (CMV) enhancer upstream of the tk promoter.
This combination confers strong transcriptional activation not only in a variety of vertebrate cell lines, but also in vivo in whole fish embryos injected with the vector DNA.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

Related Products
component of:ATCC 87654
Shipped frozen
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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