ptkLUC+ (ATCC® 87632)

Applications: reporter construct  shuttle vector  /  Depositors: J Duschl, J Altschmied

Permits and Restrictions

View Permits View Restrictions

Designations ptkLUC+
Depositors J Duschl, J Altschmied
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.7109999656677250
DESCRIPTION OF VECTOR:
Intact vector size: 4.711
Type of vector: plasmid
Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI
Polylinker sites: HindIII SalI BamHI [tk-promoter luc+ SV40 poly (A)] ClaI
SmaI KpnI SacI
Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI
XmnI SspI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: TK, 37-200
reporter group: luciferase (luc+), ->, 238-1890
terminator: SV40 late polyadenylation, 1922-2143
MCS: ClaI...SacI, 2146-2168
replicon: pMB1, 2587-2587
marker(s): ampR, <-, 3345-4205
terminator: SV40 large T-antigen polyadenylation, 4337-4708
Vector: ptkLUC+ (plasmid)
Promoters: Promoter TK
Marker(s):ampR
Construct size (kb): 4.710999965667725
Features: marker(s): ampR
promoter: TK
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...SacI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Applications
reporter construct
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

Related Products
component of:ATCC 87654
Shipped frozen
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map