pLUC+ (ATCC® 87630™)

Applications: promoter-cloning vectorreporter constructshuttle vector  /  Depositors: J Duschl, J Altschmied

Designations pLUC+
Depositors J Duschl, J Altschmied
Biosafety Level 1
Vector Information
Size (kb): 4.5529999732971190
Vector: pLUC+ (plasmid)
Marker(s):ampR
Construct size (kb): 4.552999973297119
Features: marker(s): ampR
replicon: pMB1
reporter group: luciferase (luc+)
MCS: HindIII...BamHI
MCS: XhoI...EcoRI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Applications
promoter-cloning vector
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--4.6; HindIII--4.6.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

Related Products
component of:ATCC 87654
Shipped FD
Shipping Information

 Freeze dried Escherichia coli containing the plasmid.

Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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