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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.989 Vector: YEplac112 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.989 Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
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Applications
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YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
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Comments
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This is a YE-type (yeast episomal) vector with the TRP1 marker. The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors ( ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9.
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References
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Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106
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Related Products
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component of:ATCC 87638
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Shipping Information
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Freeze dried E. coli containing the plasmid
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