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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.7410001754760740 Vector: YEplac181 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):LEU2,ampR Construct size (kb): 5.741000175476074 Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
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Applications
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YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
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Comments
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Restriction digests of the clone give the following sizes (kb): AvaI--5.2, 0.8; ClaI--6.0; EcoRI--6.0. One of 9 shuttle vectors ( ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
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References
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Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106
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Related Products
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component of:ATCC 87638
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Shipping Information
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Freeze dried Escherichia coli containing the plasmid.
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