YEpFAT10 (ATCC® 87554)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman, K Runge

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Designations YEpFAT10
Depositors DJ Stillman, K Runge
Biosafety Level 1
Vector Information
Size (kb): 6.7709999084472660
Vector: YEpFAT10 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pVZ1
Marker(s):TRP1,ampR,LEU2-d
Construct size (kb): 6.770999908447266
Features: marker(s): LEU2-d
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron ori/LEU2-d
replicon: pMB1
MCS: ApaI...SacII
MCS: NruI...SphI
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI-- 4.2, 2.9; BamHI-- 7.0; SalI-- 7.0.
Selection for TRP1 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Similarly, the NruI, BamHI, SalI and SphI are adjacent and can be used for directional cloning. The vector was constructed by Kurt Runge.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Conrad MN, et al. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63: 739-750, 1990. PubMed: 2225074

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information

 Freeze dried stock of Escherichia coli containing the plasmid.

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