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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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S. cerevisiae/E. coli pRS400 shuttle vectors
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Depositors
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P Hieter, JD Boeke, TW Christianson
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Vector Information
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DESCRIPTION OF VECTOR:
Intact vector size: 4.898
Type of vector: phagemid
Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
insert detection: lacZ', <-, 1800-2137
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184
centromere: CEN6, 4326-4442
replicon: ARSH4, 4443-4806
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References
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Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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Shipping Information
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Distributed: freeze-dried
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