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pRS416 (ATCC^® 87521^™)

Applications: YC-type (centromeric) shuttle vector / Depositors: P Hieter

pRS416 ATCC^® 87521^™

frozen

For-Profit: $290.00 Non-Profit: $290.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pRS416
Depositors	P Hieter
Biosafety Level	1
Vector Information	Size (kb): 4.898 DESCRIPTION OF VECTOR: Intact vector size: 4.898 Type of vector: phagemid Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI NotI SacII SacI NaeI Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII Construction: pBluescriptII KS+ Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): URA3, ->, 417-1219 replicon: f1, <-, 1351-1807 insert detection: lacZ', <-, 1800-2137 promoter for in vitro transcription: T7, ->, 1977-1996 MCS: KpnI...SacI, ->, 2004-2111 promoter for in vitro transcription: T3, <-, 2123-2142 promoter: lac, <-, 2212-2240 replicon: pMB1, 2566-2566 marker(s): ampR, <-, 3324-4184 centromere: CEN6, 4326-4442 replicon: ARSH4, 4443-4806 Vector: pRS416 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):URA3,ampR Construct size (kb): 4.898 Features: insert detection: lacZ' marker(s): URA3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6
Applications	YC-type (centromeric) shuttle vector
Comments	Restriction digests of the clone give the following sizes (kb): PvuII--4.45, 0.45; BglI--3.2, 1.7; PstI--3.25, 1.65. A YC-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
Related Products	component of:ATCC 87534
Shipped	FZ
Shipping Information	Distributed: freeze-dried

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector Map